scholarly journals Structure and blood supply of intrinsic lymph nodes in the wall of the rabbit urinary bladder—studies with light microscopy, electron microscopy, and vascular corrosion casting

1998 ◽  
Vol 252 (3) ◽  
pp. 477-484 ◽  
Author(s):  
Fred E. Hossler ◽  
Frederick C. Monson
Keyword(s):  
Electron Microscopy ◽  
Urinary Bladder ◽  
Lymph Nodes ◽  
Light Microscopy ◽  
Blood Supply ◽  
Corrosion Casting ◽  
Rabbit Urinary Bladder

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

A Sarcocystis-like Protozoon in a Sheep with Lymphadenopathy and Myocarditis

1978 ◽  
Vol 15 (2) ◽  
pp. 186-195 ◽  
Author(s):  
T. Landsverk ◽  
H. Gamlem ◽  
R. Svenkerud
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Light Microscopy ◽  
Vascular Endothelial Cells ◽  
Protozoan Parasite ◽  
Capillary Endothelium ◽  
Vascular Endothelial ◽  
Generalized Lymphadenopathy

A generalized enlargement of the lymph nodes was found in an emaciated adult ewe. Additional autopsy findings included tiny grey-white necrotic foci in the heart muscle, aspiration pneumonia and diffuse pleuritis. Light microscopy showed a generalized lymphadenopathy with Perilymphadenitis, depletion of lymphocytes and histiocytosis of the lymph node. In histiocytes and vascular endothelial cells of lymph nodes, septal capillary endothelium of lungs and capillary endothelium of myocardium, early stages of a protozoan parasite were found. In the myocardium, there were many foci of necrosis, some of which contained young cysts in the periphery. These cysts were morphologically similar to those of Sarcocystis. Electron microscopy of the early protozoan stages yielded evidence of schizogony and formation of merozoites.

scholarly journals Microvascular Architecture of the Mouse Urinary Bladder Described with Vascular Corrosion Casting, Light Microscopy, SEM, and TEM

2009 ◽  
Vol 15 (S2) ◽  
pp. 984-985 ◽  
Author(s):  
F Hossler ◽  
A Lametschwandtner ◽  
R Kao ◽  
C Bills ◽  
F Finsterbusch
Keyword(s):  
Urinary Bladder ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Sem And Tem ◽  
Microvascular Architecture

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

Blood Supply and Function of the Tegmentum Vasculosum (Stria Vascularis) of the Duckling Cochlea

1999 ◽  
Vol 5 (S2) ◽  
pp. 1194-1195
Author(s):  
F.E. Hossler
Keyword(s):  
Electron Microscopy ◽  
Blood Supply ◽  
Carotid Arteries ◽  
Stria Vascularis ◽  
Light And Electron Microscopy ◽  
Corrosion Casting ◽  
The Common ◽  
And Function ◽  
Tegmentum Vasculosum ◽  
Common Carotid Arteries

On the basis of ultrastructural, biochemical, cytochemical, and electro-physiological evidence, the stria vascularis, a highly vascular epithelium of the mammalian cochlea, has been implicated as one site of endolymph synthesis. It would seem natural to suspect that its highly developed counterpart in the avian cochlea, the tegmentum vasculosum (TV), might have a similar function. This study utilizes routine light and electron microscopy, biochemistry, cytochemistry, and vascular corrosion casting to describe the structure and blood supply to the (TV) in the duckling and provide evidence for its involvement in endolymph synthesis.Four to 16 week-old ducklings (Anas platyrhyncos)were anesthetized and anticoagulated and perfused via the common carotid arteries with buffered, 2% glutaraldehyde for routine light microscopy or transmission or scanning electron microscopy, or with Mercox resin for vascular corrosion casting. Casts were macerated in 5% KOH and warm water to remove tissue, cleaned in formic acid and water, dried by lyophilization, mounted on stubs and viewed at 10-20 kv by SEM.

Microvascular Architecture of Mouse Urinary Bladder Described with Vascular Corrosion Casting, Light Microscopy, SEM, and TEM

2013 ◽  
Vol 19 (6) ◽  
pp. 1428-1435 ◽  
Author(s):  
Fred E. Hossler ◽  
Alois Lametschwandtner ◽  
Race Kao ◽  
Friederike Finsterbusch
Keyword(s):  
Urinary Bladder ◽  
Light Microscopy ◽  
Collateral Circulation ◽  
Normal Function ◽  
Genetic Models ◽  
Corrosion Casting ◽  
Sem And Tem ◽  
Capillary Plexus ◽  
Human Disorders ◽  
Microvascular Architecture

AbstractThe urinary bladder is a unique organ in that its normal function is storage and release of urine, and vasculature in its wall exhibits specialized features designed to accommodate changes in pressure with emptying and filling. Although we have previously described the fine details of the microvasculature of the urinary bladder of the rabbit and dog, information on the fine details of the microvasculature of the mouse bladder were deemed to be of value because of the increasing use of this species in developing genetic models for studying human disorders. The present study shows that many of the special features of the microvasculature of the mouse urinary bladder are similar to those described in the rabbit and dog, including vessel coiling, abundant collateral circulation, arterial sphincters, and a dense mucosal capillary plexus.

Early-stage microvascular alterations of a new model of controlled cortical traumatic brain injury: 3D morphological analysis using scanning electron microscopy and corrosion casting

2013 ◽  
Vol 118 (4) ◽  
pp. 763-774 ◽  
Author(s):  
Simone Sangiorgi ◽  
Alessandro De Benedictis ◽  
Marina Protasoni ◽  
Alessandro Manelli ◽  
Marcella Reguzzoni ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Traumatic Brain Injury ◽  
Scanning Electron Microscopy ◽  
Brain Injury ◽  
Light Microscopy ◽  
Morphological Characteristics ◽  
Corrosion Casting ◽  
Brain Swelling ◽  
Microvascular Alterations ◽  
Scanning Electron

Object This study was performed to study the microvascular changes that occur during the first 12 hours after traumatic brain injury (TBI) using the corrosion casting technique. Methods The authors performed a qualitative and quantitative morphological study of the changes in cerebral vessels at acute (3 hours) and subacute (12 hours) stages after experimental TBI. They used a model of controlled cortical impact (CCI) injury induced by a recently developed electromagnetic device (impactor), focusing their observations mainly on the microvascular alterations responsible for the formation and maintenance of tissue edema and consequent brain swelling during the first hours after TBI. They used corrosion casting, scanning electron microscopy (SEM), light microscopy, and transmission electron microscopy (TEM) to obtain a morphological qualitative map with both 2D and 3D details. Results Scanning electron microscopy analysis of vascular casts documented in 3 dimensions the typical injuries occurring after a TBI: subdural, subarachnoid, and intraparenchymal hemorrhages, along with alterations of the morphological characteristics and architecture of both medium-sized and capillary vessels, including ectasia of pial vessels, sphincter constrictions at the origin of the perforating vessels, focal swelling of perforating vessels, widening of intercellular junctions, and some indirect evidence of structural impairment of endothelial cells. All of these vascular alterations were confirmed in 2D analyses using light microscopy and TEM. Conclusions The corrosion casting–SEM technique applied to a CCI experimental model proved to be a reliable method for studying the pathophysiology of the vascular alterations occurring at acute and subacute stages after CCI injury. It was also possible to obtain topographical localization of the vascular and cellular events that usually lead to hyperemia, edema, and brain swelling. Moreover, by applying informatic software to anatomical images it was possible to perform quantification and statistical analysis of the observed events.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

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